Modifying Enzymes
Zymo Research Corporation dsDNA Shearase™ Plus 50 U (1 U/ul)
dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs, thus minimizing sample loss.
New England Biolabs, Inc. T4 Phage beta-glucosyltransferase – 500 units
T4 Phage -glucosyltransferase specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA, making beta-glucosyl-5-hydroxymethylcytosine.
New England Biolabs, Inc. Bst DNA Polymerase, Large Fragment – 8000 units
Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5' to 3' polymerase activity, but lacks 5' to 3' exonuclease activity.
AVIDITY LLC BirA biotin-protein ligase bulk reaction kit
The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5’-adenylate) in a highly efficient and targeted manner. This kit includes 300 µg of BirA enzyme for those who require larger scale reactions or more frequent use of the BirA biotinylation reaction in their work.
Rockland Immunochemicals Lysozyme (Hen Egg White) - MB-109-1000
Lysozyme is a from a family of enzymes that act upon glycoproteins in the cell walls of bacteria. Lysozyme is a highly abundant protein in a variety of secretions: tears, saliva, milk, and mucus (in mammals). The whites of avian eggs are also an abundant source of lysozyme.
Zymo Research Corporation DNA Degradase™ (500 U)
DNA Degradase™ is ideal for whole-genome DNA methylation analysis by a number of downstream applications (i.e., HPLC, TLC, etc.). Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.DNA Degradase™degrades DNA to individual nucleotides.Quantitation of nucleotides to detect DNA methylation levels can be assessed by various applications (e.g.- TLC, HPLC, etc.). For detection of DNA methylation via LC/MS, DNA needs to be degraded down to individual nucleosides.
Cayman Chemical Coenzyme Q10 100mg
A cofactor in the electron transport chain; decreased levels due to genetic mutations in its synthesis enzymes lead to encephalopathy, cerebellar ataxia, infantile multisystemic form, nephropathy, and isolated myopathy
Sigma Aldrich Fine Chemicals Biosciences alpha-Amylase Bacillu250ML
Amylase belongs to glycosyl hydrolase family 13. It comprises of A B and C domains. Domain A has two aspartic acid and a glutamic acid as catalytic residues. Domain B possesses Ca2 and Na binding sites.
Enzo Life Sciences Ubiquitin activating enzyme E1 (human), (recombinant) (His-tag) (50 µg)
Essential ubiquitinylation pathway enzyme. MW: 118 kDa. Purity: ≥90% (SDS-PAGE). Formulation: In 50mM TRIS-hydrochloric acid, containing 1.0mM dithiothreitol. Source: Produced in E. coli BL21 (λDE3) expression system and containing a C-terminal His6-tag. Full length human ubiquitin-activating enzyme E1. UniProt: P22314. Handling: Avoid freeze/thaw cycles. Long Term Storage: -80°C
Sigma Aldrich Fine Chemicals Biosciences Deoxyribonuclease I from bovine pancreas Type IV, lyophilized powder, >=2,000 Kunitz units/mg protein | 9003-98-9 | MFCD00130918 | 375KU
Deoxyribonuclease I from bovine pancreas Type IV, lyophilized powder, >=2,000 Kunitz units/mg protein | 9003-98-9 | MFCD00130918 | 375KU
C LECTA GMBH DENARASE 5000 kU, R&D Enzymes & Nucleases
For process development of biologicals such as viral vectors and vaccines The enzyme cleaves all forms of DNA and RNA and enables a simpler more efficient downstream process - Purity 99% - Activity 250 U/ul - Formulation buffered aqueous glycerol solution- Storage -20C - Produced in conformity with ISO 9001 standard- Manufacturing free of animal-derived raw materials and antibiotics- Depending on your application 10-100 U/ml is needed - Net price excluding VAT and shipment costs
c-LEcta will ship the Products, as the case may be and at its sole discretion, either directly from Leipzig, Germany, or from c-LEcta’s third party service provider in the US. In the latter case, c-LEcta has already incurred all costs and fees related to the import of the Products into the US. Such costs and fees will be passed on by c-LEcta to the customer without any deductions or surcharges, if the Products are shipped from c-LEcta’s third party service provider in the US to the customer
New England Biolabs, Inc. EnGen Lba Cas12a (Cpf1) – 2000 pmol
EnGen Lba Cas12a from Lachnospiraceae bacterium ND2006 is a site-specific DNA endonuclease guided by a single 41-44 nucleotide guide RNA (gRNA) . Targeting requires a gRNA complementary to the target site as well as a 5' TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by EnGen Lba Cas12a occurs ~18 bases 3' of the PAM and leaves 5' overhanging ends. EnGen Lba Cas12a has Simian virus 40 (SV40) T antigen nuclear localization sequences (NLS) at both the N and C-termini of the protein.
Cayman Chemical Oleoyl SerInol 50mg
An analog of ceramide and a GPR119 agonist (EC50 = 12 µM); induces cell death in F-11 murine neuroblastoma and U-87 human astrocytoma cells at 100 µM; induces apoptosis in rapidly dividing cells and prevents teratoma formation in a neonatal mouse engraftment model of teratoma formation
Cayman Chemical MaLNyl Coenzyme AlIthIM1mg
A CoA derivative used in fatty acid and polyketide synthesis and in the transport of α-ketoglutarate across the mitochondrial membrane; may mediate cancer cell cytotoxicity induced by fatty acid synthase inhibition
Apexbio Technology LLC Lambda Exonuclease(Synonyms: Lambda Exo, Lambda exonuclease enzyme, λ exonuclease, λ exonuclease enzyme), 5000U.
Lambda Exonuclease is a DNA-specific exonuclease enzyme that progressively degrades double-stranded DNA in a 5 3 direction preferentially initiating digestion at 5 -phosphorylated termini It displays minor activity toward single-stranded DNA and double-stranded DNA with non-phosphorylated ends but cannot start cleavage at internal nicks or gaps Lambda Exonuclease is commonly employed in molecular biology laboratories for the generation of single-stranded DNA templates DNA single-strand conformation polymorphism (SSCP) analysis selective degradation of linear plasmid forms while preserving supercoiled structures and rolling-circle amplification protocols