Modifying Enzymes
STEMCELL Technologies Elastase, Size: 25 mg
Elastase is a serine protease consisting of a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges (Shotton & Hartley). Elastase is synthesized as a zymogen (proelastase) and is converted to the active form by limited trypsin proteolysis at its N-terminal. Elastase preferentially cleaves the peptide bond of C-terminal neutral, non-aromatic amino acid residues (Schellenberger et al.). Elastase is frequently used in combination with other proteases such as collagenase and trypsin to digest fibrous tissues because of its ability to digest native elastin.
Enzo Life Sciences Cathepsin D (human liver), (purified) (25 µg)
Purity: ≥95% (SDS-PAGE). Formulation: Lyophilized with phosphate buffer. Source: Isolated from human liver. Long Term Storage: -80°C.
Enzo Life Sciences MMP-8 (catalytic domain) (human), (recombinant) (10 µg)
Alternative name: Matrix metalloproteinase 8, Neutrophil collagenase, Collagenase 2. Purity: ≥90% (SDS-PAGE). Formulation: Liquid. In 50mM TRIS, 5mM CaCl2, 300mM NaCl, 20µM ZnCl2, 0.5% Brij-35, and 30% glycerol. Source: Produced in E. coli. Active Matrix Metalloproteinase-8 (MMP-8, neutrophil collagenase, collagenase-2) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-8 (Phe99-Gln269, NM_002424) with a C-terminal purification tag. This represents a naturally-occurring active form of MMP-8 which lacks the C-terminal hemopexin domain1. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin, casein, or peptide substrates is unaffected. Long Term Storage: -80°C.
Macherey-Nagel Proteinase K (100 mg)
Proteinase K (100 mg) lyophilized Proteinase K incl. Proteinase Buffer pack of 100 mg
LGC GENOMICS LLC (Lucigen) NxGen M-MuLV Reverse Transcriptase, 50,000 UOutstanding cDNA synthesis.
Supplied at 200,000 U/mL in 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% NP-40 Alternative, 50% glycerol, pH 7.6 @ 25 C. Also provided is 10X M-MuLV RT Buffer which in 1X form is composed of 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25 C. 250,000 U is supplied as 5 50,000 U.
Omega Biotek Inc Proteinase K, 10 mL solution, 20 mg/mL concentration, non-specific serine protease, RNase/DNase free, high stability in SDS, EDTA, urea
Omega Bio-tek Proteinase K is a non-specific serine protease that is essential for various molecular biology applications. Supplied as a ready-to-use 10 mL solution, it effectively inactivates nucleases and promotes cell lysis, ensuring high-quality DNA and RNA isolation. This enzyme is stable across a broad pH range and maintains its activity even in the presence of protein-denaturing agents such as SDS, EDTA, and urea.
- Volume: 10 ml
- Formulation: Ready-to-use solution
- Concentration: 20 mg/ml
- Activity: >600 mAU/mg
- Enzyme type: Non-specific serine protease
- Source organism: Tritirachium album
- Stability: High stability in SDS, EDTA, urea
- pH range: Wide range
- Primary application: Inactivation of nucleases and lysis for DNA/RNA isolation
- Purity: RNase/DNase free
- Storage temperature: -20°C
- Shipping condition: Requires ice pack
- Country of origin: USA
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline-Agarose from calf intestine ammonium sulfate suspension | MFCD00131852 | 100UN
Phosphatase, Alkaline-Agarose from calf intestine ammonium sulfate suspension | MFCD00131852 | 100UN
New England Biolabs, Inc. SacI-HF – 2000 units
A high fidelity restriction endonuclease that recognizes the sequence G_AGCT^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. USER® Enzyme – 250 units
USER (Uracil-Specific Excision Reagent) Enzyme generates a single nucleotide gap at the location of a uracil. USER Enzyme is a mixture of Uracil DNA glycosylase (UDG) and the DNA glycosylase-lyase Endonuclease VIII. UDG catalyses the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease VIII breaks the phosphodiester backbone at the 3 and 5 sides of the abasic site so that base-free deoxyribose is released.Applications of USER include:
- Directional RNA Seq
- NEBNext adaptor cleavage
- USER cloning
New England Biolabs, Inc. E.coli DNA Ligase – 200 units
E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated.
New England Biolabs, Inc. T4 DNA Ligase – 100,000 units – 2,000,000 units/ml
The industry standard for performance and quality. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA
- Joins blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA
- T4 DNA Ligase variants: Salt-T4, Hi-T4, Instant Sticky-end Ligase MM, Blunt/TA Ligase MM
Andwin Scientific SNAP STRP NAT.PCR TBS 120 OF 8
The 0.2 mL GeneMate SnapStrip 8-Strip Standard PCR Tubes feature individually attached dome caps and ultra-thin wall that permits efficient heat transfer. This product conveniently allows users to construct a strip of less than 8 tubes with caps by simply breaking the strip where desired. It is sold in a natural color in a quantity of 120.
Sigma Aldrich Fine Chemicals Biosciences beta-Glucuronidase from limpets (Patella vulgata) aqueous solution | 9001-45-0 | MFCD00130629 | 10ML
beta-Glucuronidase from limpets (Patella vulgata) aqueous solution | 9001-45-0 | MFCD00130629 | 10ML
